| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052038 |
| Chromosome: | chromosome 12 |
| Location: | 7440933 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557000 | ATG12,APG12 | Autophagy-related protein; (1 of 1) K08336 - ubiquitin-like protein ATG12 (ATG12) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGAGGCAGTCTTGAGATGTTTGTCAGTTGTTAAAGCAAAGCGGCGCC |
| Internal bar code: | GTCAAGGTCGGGCTTGTCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 31.5789 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATCAGACCCTTCCACCC |
| Suggested primer 2: | ACGGAGTACACCCAGGCTAT |