| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052063 |
| Chromosome: | chromosome 16 |
| Location: | 1514340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATTAACTTTAATATTTTAGGGGGTCAAGGCAATGGGGGCGCTTGCGC |
| Internal bar code: | AAGTAAAAACATTGATGCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3481 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGGCTAGCGTCATCGT |
| Suggested primer 2: | GCGCTTCTGCTTGTGTGAAA |