| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052064 |
| Chromosome: | chromosome 6 |
| Location: | 2984171 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273950 | HFO7 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGGGCTTGGTGATGCCCTGGATGTTGTCGCGAAGCACCTTGCGGTGG |
| Internal bar code: | TGTATTTATTTGAGACCCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2851 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGGGCATGATGGTCAC |
| Suggested primer 2: | CGCCCTTCGTATGCTCTGAT |