Insertion junction: CLIP2.052072_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.052072
Chromosome:chromosome 9
Location:518319
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g748747 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTGTCTGTTTCAGCACGAAGTGCATTTGCGCACCAGTGCTCTTTTGG
Internal bar code:GCTCGGACCATACTGGCAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:575
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGCTCAACACACTGCTG
Suggested primer 2:TAGGATTGGGACCGGGACTT