| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.052087 |
| Chromosome: | chromosome 2 |
| Location: | 7343192 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391726 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTGCAAAAGGGTTGGTGGGTCAAGGGGCATGAGCGCATTGCGAATG |
| Internal bar code: | CGATCTAGCCTAGAAGCTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTGAGGACTTCGCGATG |
| Suggested primer 2: | TGTCGGTGTAGCGACGAAAA |