| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052130 |
| Chromosome: | chromosome 16 |
| Location: | 6455113 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675000 | HEMD,UROS2,UPS3,UROS,UPS2 | Uroporphyrinogen-III synthase; (1 of 2) PF02602 - Uroporphyrinogen-III synthase HemD (HEM4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAACCGGCAAGCGGACTTCGGCCATCTCTTGACACTGGCTGGCCTAAT |
| Internal bar code: | GTACAATGTATATGGCAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3527 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGACAGAGGTGAGCGTG |
| Suggested primer 2: | ACAAGAGTGTGGCAGGTACG |