| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052138 |
| Chromosome: | chromosome 14 |
| Location: | 1916561 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620250 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGAGGAGCTCTCCGCCGCCGCCACCGCTTGCCGCCTGGTCGTGACAC |
| Internal bar code: | AATATAGGGGGTTAGGGATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4910 |
| LEAP-Seq percent confirming: | 91.0448 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGCGCTACAGGAATTGC |
| Suggested primer 2: | CTGTGTTATACCGCGCGTTG |