Insertion junction: CLIP2.052147_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052147
Chromosome:chromosome 9
Location:3217004
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394658 LCI28 (1 of 1) 1.1.1.21 - Aldehyde reductase / Polyol dehydrogenase (NADP(+)); Low-CO2-induced aldose reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACGACCCCTAAGGCAGTGGCCCGAAGTTCTCAACCGACGCTTTGCTG
Internal bar code:TTTGCCTGTATGGTATACAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAGATAGGACACCTGGGG
Suggested primer 2:CAACACAAATGCCGACACGT