| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052154 |
| Chromosome: | chromosome 6 |
| Location: | 8017918 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304800 | (1 of 1) PTHR11216//PTHR11216:SF61 - EH DOMAIN // GH06923P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGGCCAGGTGCGGAGCTGGAGCAAGAGTGGGCAAATGACAGCATGC |
| Internal bar code: | TAAGCTTAGGAAGGGTAGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1280 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGCAGGTGGGAGGTTC |
| Suggested primer 2: | AAGGTGCGGTGTATCCAAGG |