| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052156 |
| Chromosome: | chromosome 16 |
| Location: | 1874644 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655550 | CDS | ||
| Cre16.g655600 | (1 of 4) PF16016 - Domain of unknown function (DUF4782) (DUF4782) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTGCTGTTGCAGAAGCCGCTCCCCGACCGCCGCCCCGTCCTGTTCGC |
| Internal bar code: | GAGTCGTTGGGGGAGTGTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3636 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGGTGGCAAGGCTGTTC |
| Suggested primer 2: | GCCACACGTCAACAACTTCC |