| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052157 |
| Chromosome: | chromosome 2 |
| Location: | 6014477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118700 | (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | intron | |
| Cre02.g800246 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCCTACCATTCCTTCCCTCACCCTCCTCCACAGCCTGGCTTACATC |
| Internal bar code: | GTCGCTAGTTTGAGATAACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1633 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACGAGGAAGAGGACGATG |
| Suggested primer 2: | GGCTTGCAGTCAACGACAAG |