Insertion junction: CLIP2.052159_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.052159
Chromosome:chromosome 12
Location:2023839
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g510600 STK11,STPK11 Serine/threonine protein kinase; (1 of 1) 2.7.11.1//2.7.11.21 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Polo kinase / Polo-like kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGGACGAGTCTGAAGCGGCGAGCTTTAGCGCCACCGACAGCGGGGA
Internal bar code:TCTGCGCCCATTTCGATAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:431
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACACCACCATCACCACC
Suggested primer 2:ATGCCCTGCAAGATTGAGCT