| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052164 |
| Chromosome: | chromosome 5 |
| Location: | 2291708 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238150 | (1 of 1) PF07646 - Kelch motif (Kelch_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCGCGATGCATAGTAGGACCGCCTGACCCTTCCCAGGAGCCCAGGAG |
| Internal bar code: | GGCATATTACGGCAAGTGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 48.8889 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCCTTGCCACGTCTTGA |
| Suggested primer 2: | CAAGTACTCCATGTTGCGCG |