| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.052180 |
| Chromosome: | chromosome 1 |
| Location: | 4181627 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGGGAGGCGCTGACGACCAACGGCGCTGACGGCACTGCTGGTGCGG |
| Internal bar code: | GAAAAGTGTGCACACTCTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6262 |
| LEAP-Seq percent confirming: | 69.4915 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTTACACTTCTGCACT |
| Suggested primer 2: | CCATCTGTCCCAGCTGCTAG |