| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052195 |
| Chromosome: | chromosome 12 |
| Location: | 9447221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGGATGCAGGAGCTGCAGCGGCTGCCCCATTCGGTGCCCATTGTGTA |
| Internal bar code: | GATGTGCATAGGATCGCCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3856 |
| LEAP-Seq percent confirming: | 58.6207 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTTGCTTTTGTTCTCGC |
| Suggested primer 2: | CAGAACGTAAGGCCCAGTGT |