| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052200 |
| Chromosome: | chromosome 10 |
| Location: | 1855055 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431050 | SRR13 | (1 of 1) PTHR19331//PTHR19331:SF253 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED; Speract/scavenger receptor, transmembrane glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTTTCCTCCCCACCCGCCACCGGGGCACCCACCTTCACCGCGGCCGC |
| Internal bar code: | TGCGATTTTGTCGTAGAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2499 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGTCAGGCATGTTACGA |
| Suggested primer 2: | CCCTGTTACCCATGCCCAAT |