| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.052207 |
| Chromosome: | chromosome 3 |
| Location: | 5154549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181000 | (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTCAGGGAAGACGGAGGTAATAGACACAGCAAGATGAGCATGGCTC |
| Internal bar code: | CGCACAAGAGATTTTTGTAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4996 |
| LEAP-Seq percent confirming: | 84.7222 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAACGCCCCCGAAATATA |
| Suggested primer 2: | CATGGTTTGTTTGACGGCGT |