Insertion junction: CLIP2.052210_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052210
Chromosome:chromosome 3
Location:1271828
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g149950 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACATGGATTGAAGACCCCGGGCTCCGCCAGGGCTGGAACGTCACGTA
Internal bar code:TATGAGCACATGCGGTTGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:85.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GACTACCGTAGCAGTCTCGC
Suggested primer 2:TCCAGGTAAACACACCTCGC