Insertion junction: CLIP2.052233_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052233
Chromosome:chromosome 1
Location:6150455
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g043750 BBS7 (1 of 1) K16749 - Bardet-Biedl syndrome 7 protein (BBS7); Bardet-Biedl syndrome-7 associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGGCAAGGGTAGTCGCAACAGTACGGTGTGCAAAGGATTTGGTCTTG
Internal bar code:TATGGGTCTTGGAACGAACAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:697
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTAACCTCAGGTGCTGCC
Suggested primer 2:ACAGGATAGGGCTGTACGGT