Insertion junction: CLIP2.052236_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052236
Chromosome:chromosome 13
Location:3842758
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTACTTCCCCACGCGCAGGTTCCGCGCCGTGTTTGGAGACCGCGAGGT
Internal bar code:TTCATGGGCGATTTTTTTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1356
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGGACGTAGGTCACCT
Suggested primer 2:CTGGTGGACGAGGGACTAGA