| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052261 |
| Chromosome: | chromosome 14 |
| Location: | 1754657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619200 | (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGCGAAAGGCAGCATTCCTGTTTGGGTGTGCACGCGCAGGCATGAG |
| Internal bar code: | GATCGAGCGGCGTAACGGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1909 |
| LEAP-Seq percent confirming: | 91.0714 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTTCGAGGCCTTGCAGA |
| Suggested primer 2: | CATCGCGTCATCCAGGATGA |