| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052267 |
| Chromosome: | chromosome 16 |
| Location: | 3834202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688650 | MOT43 | (1 of 14) PF02493 - MORN repeat (MORN); Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAGGCCCGTCCACCCCTGTCTCCACCCCATCCTCCGGCTCCGCCCCC |
| Internal bar code: | ACCTTCGGACGATGCGTGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1336 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCAGCCTTCCTCTAGA |
| Suggested primer 2: | TCCCTGACGATATGCCATGC |