| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052275 |
| Chromosome: | chromosome 12 |
| Location: | 2695720 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| lncRNA_TCONS_00077901 |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGACCCAACCCTGACGACCCAACCCTGACGACCCAACCCTGCCCCA |
| Internal bar code: | CAGTATGTGATATAGCGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 87.0968 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAATGATGTCATTCGCCC |
| Suggested primer 2: | CACAGCCACCAACACACATG |