Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.052279 |
Chromosome: | chromosome 6 |
Location: | 2120356 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g266250 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGACCTGGTGCACCGCGCGGACGTGGTCAAGCGCTTCGTCGCCAAGAA |
Internal bar code: | TTTGTCACGCTCGGGCCGGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4275 |
LEAP-Seq percent confirming: | 3.33333 |
LEAP-Seq n confirming: | 2 |
LEAP-Seq n nonconfirming: | 58 |
LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTACGTCCCTCGATCTTGC |
Suggested primer 2: | GCTTGCCTCCTCTGCAGTTA |