Insertion junction: CLIP2.052282_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.052282
Chromosome:chromosome 7
Location:5228199
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g349137 CDS
lncRNA_TCONS_00101497 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGTGCCCCGTTCCAGAAAGCCTCCATCGCATTGAAAACCGCTGCTGTC
Internal bar code:CAGACGTTAGAAGTGGGGAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1339
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTATGGCGCATGTGACAG
Suggested primer 2:CTGCTATCGGGAGGCATCAG