Insertion junction: CLIP2.052296_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052296
Chromosome:chromosome 16
Location:284586
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g694200 MMP15 Matrix-metalloproteinase-like protein; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCTCCCCCACCTCCCCACCATCCCACCACCAGACGGCGGCCCCTGTA
Internal bar code:GCATCGGTCAGACTGTTTCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:233
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAGACTGTGCAGAGTCC
Suggested primer 2:GCAGTAGCAGTAGCAGCAGT