| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.052303 |
| Chromosome: | chromosome 7 |
| Location: | 3937731 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCAGCATAGCTTGAGCCGCCCACTCAAGGCTAGCCATATTCGCACA |
| Internal bar code: | TTCTGGTTTCGTATAGAGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1271 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCGCTTTTGACGTTAGA |
| Suggested primer 2: | GGAATCGTGGAAAGGAGGCA |