Insertion junction: CLIP2.052335_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.052335
Chromosome:chromosome 13
Location:4604142
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g603600 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTTGACCAGCACGCTGTCGTTGATGTACAGCAGCGGCCGCAGGGCCTC
Internal bar code:CAAACCTTTCGTGGGAGGCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2543
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTGTGCTCTTTCGTTTG
Suggested primer 2:AATCGCACCCATCCACACAT