| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052355 |
| Chromosome: | chromosome 12 |
| Location: | 3849362 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515050 | HLM18,SET4 | putative N-methyltransferase; (1 of 1) PTHR13793:SF5 - HISTONE-LYSINE N-METHYLTRANSFERASE ATX4-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGGCGGTGCGGTGGGAGGAGCGGCTGCAGCGGGCGCTGGCGACGGA |
| Internal bar code: | CCGTGTGGACACTAAATAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3623 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTCGGTCGTTTGTGTG |
| Suggested primer 2: | ACCAGCATTGAACCTAGGGC |