| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052360 |
| Chromosome: | chromosome 16 |
| Location: | 5083131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685650 | GLR1 | Ionotropic glutamate receptor; (1 of 4) PF00060//PF00497 - Ligand-gated ion channel (Lig_chan) // Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAAACCCACAGCGCCGCACCTACCTGGATGTTGACGGCTGTCAGGCG |
| Internal bar code: | ACAGGGTCGAATGAGGTCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 42.3077 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTGTCACTTGTGCCGC |
| Suggested primer 2: | GCAGTTTGCAGCATCACACA |