| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052378 |
| Chromosome: | chromosome 3 |
| Location: | 6251612 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191700 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGAATCTTCGGAGGGGCAACATGCCTTGCGTGTTAAGGCATGGCTTTA |
| Internal bar code: | AACGGTGAGGTAATGAAGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2003 |
| LEAP-Seq percent confirming: | 91.4286 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAGTGTGTGCGTCGCATG |
| Suggested primer 2: | CTGACTAGGGACACGCCATG |