| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052383 |
| Chromosome: | chromosome 6 |
| Location: | 5055624 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282250 | NAT18 | N-acetyltransferase; (1 of 1) 2.3.1.48//2.3.1.88 - Histone acetyltransferase // Peptide alpha-N-acetyltransferase / Protein N-terminal acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACAAAGCCGCTGTGCTTCTCCCACACCCAGCACCCTACCTGCCTCGC |
| Internal bar code: | ACTGTACAAGGTTGTCCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1273 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCCTCAAGCAGTACAA |
| Suggested primer 2: | ATGTTGTAACGGGTCCGCAT |