Insertion junction: CLIP2.052399_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.052399
Chromosome:chromosome 2
Location:5546265
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g115050 RSEP3,RSE3 (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACTCCAACCGCCACCCAACGGCACACACACACACACGCACACACACAC
Internal bar code:ACCGTTTTATAAATGAGCGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4820
LEAP-Seq percent confirming:97.9592
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGTGCCTCACAACTCTT
Suggested primer 2:CACTGCCGGCCTTTACCTTA