| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.052412 |
| Chromosome: | chromosome 10 |
| Location: | 4156323 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448900 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGTCGTCCGTTTCCCCTTGAAATAGCTACTAGCTGCCATGATACTA |
| Internal bar code: | GGGAAGAGAATTCTTCACGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1627 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTACCATGCTCACCCTGC |
| Suggested primer 2: | ACATAAGGCGGTGACTGGTG |