| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052414 |
| Chromosome: | chromosome 1 |
| Location: | 3552733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023150 | MCM5 | (1 of 1) K02209 - DNA replication licensing factor MCM5 (MCM5, CDC46); Minichromosome maintenance protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAATCCCCATGTCCCGACGCGGCAGTCCAGTCTCTCACCTGCACCA |
| Internal bar code: | TTAGCCAGACCGTCCGGTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2237 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCCGCTTCAAGTTCAC |
| Suggested primer 2: | CACCATGTGTGCAGTGTGTG |