Insertion junction: CLIP2.052421_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052421
Chromosome:chromosome 7
Location:4195651
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g342050 NPSN1,VTI3 (1 of 1) K08494 - novel plant SNARE (NSPN); Endosomal Qb-SNARE, Npsn-family (plant/protist-specific) (Qb.III.d) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGGGCGCCTGGGGCAAGCGGCCAACCGCCTGCATTACTGCAACTTCG
Internal bar code:TGTTATGTAAGGGGGGCTGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:949
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTTCTTGGTCTGGTCGT
Suggested primer 2:GTGCAGGCTCATCAAGGACT