Insertion junction: CLIP2.052428_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052428
Chromosome:chromosome 12
Location:1417709
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g488600 (1 of 1) IPR000104//IPR006059 - Antifreeze protein, type I // Solute-binding family 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGGGACCCGTCCTCGGGGCCAACACGACGTCGGGTTCTCCGCGGGCGT
Internal bar code:AATATCCATTGTTAGGATTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1877
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCAAGTCCGGACAAGA
Suggested primer 2:CCAACACCGCTTCCAAAGTG