| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052430 |
| Chromosome: | chromosome 6 |
| Location: | 5424135 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285700 | (1 of 1) PTHR13466:SF0 - PROTEIN F55C12.5, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCTGCCGCCGTGGCGCAGAGACTGTTGCGCCAGGAGCGAAGGCGGC |
| Internal bar code: | GGGCTTACCCCGCCGAGGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCACGACTTGGTCTGGA |
| Suggested primer 2: | CGAACAGGTACAGGGTGGTC |