| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.052439 |
| Chromosome: | chromosome 12 |
| Location: | 3448666 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497450 | FAP412 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype; Flagellar Associated Protein 412 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGATGCCTCACAAACCTGGACATATCAAATAACCCAATCACAAGCAT |
| Internal bar code: | TATAGATGTGTATATTAGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1043 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACTTGTTAGCCCCAGCA |
| Suggested primer 2: | CGGGTGGATCGATAACCAGG |