Insertion junction: CLIP2.052450_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.052450
Chromosome:chromosome 7
Location:5341158
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350100 (1 of 1) IPR000104//IPR006311//IPR017956//IPR026741//IPR026937//IPR027417 - Antifreeze protein, type I // Twin-arginine translocation pathway, signal sequence // AT hook, DNA-binding motif // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATTCACACCCACGCACCACCACCCCTCCTCCCCTCACCGCGTCCATGC
Internal bar code:TTGGCGCGTGATGTGTGGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:200
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TATGTGGGAGGGTGGAGGAG
Suggested primer 2:AGGATGTGTGGAATGCACGT