Insertion junction: CLIP2.052453_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.052453
Chromosome:chromosome 1
Location:6281493
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g044600 (1 of 2) PF17047 - Synaptotagmin-like mitochondrial-lipid-binding domain (SMP_LBD) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGACCCCAACCACCACAGCAAGCTCATCCCCGTGAACTGGGTCAAGT
Internal bar code:AGTGGTCATATTGTGCCGGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3883
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGCGTTCAGTACCATTCC
Suggested primer 2:ACTGAACTGCATCCCTGTCG