| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052464 |
| Chromosome: | chromosome 3 |
| Location: | 629503 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145547 | CBR1 | (1 of 3) K00326 - cytochrome-b5 reductase (E1.6.2.2); NADH-cytochrome b5 reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAGGAAAGGACACGCGGCGGTCAACCAGCCGGACCAGCCACGCGATC |
| Internal bar code: | CTTGGGATAGCGGGGTCAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2388 |
| LEAP-Seq percent confirming: | 89.2308 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATATTGTGCGGTGCTGGA |
| Suggested primer 2: | TCTCACCGAAGTGCTTGACC |