Insertion junction: CLIP2.052473_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052473
Chromosome:chromosome 7
Location:3476201
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g335800 PIH1D3,TWI1,DNAAF6,Twister Twister-like Dynein assembly factor; (1 of 1) PTHR21083//PTHR21083:SF0 - TWISTER // PROTEIN PIH1D3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACCGTTCTGTACGGAACCTAGACCCCGTAACCCCATCAATGGCTCAC
Internal bar code:TGATAGGTCGTCTTCCGGGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4722
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGTTACCGCTCAGCTACG
Suggested primer 2:AGAGACGACACGAACCCAAC