| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.052492 |
| Chromosome: | chromosome 13 |
| Location: | 4196654 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591501 | PUS2 | (1 of 2) PTHR11142:SF0 - TRNA PSEUDOURIDINE SYNTHASE-LIKE 1; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCAGCGCCCCTCTGCATCTCCCTTGCCACCTCCCTTGCTGCCAGTTGC |
| Internal bar code: | GGAGCGGTCGAGAGCCGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCATTGCTTGGCTGAT |
| Suggested primer 2: | TGAACAAAACGGCAGCATGG |