| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052509 |
| Chromosome: | chromosome 6 |
| Location: | 2370697 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268200 | TGD1 | Lipid transfer machine permease; (1 of 1) PTHR30188//PTHR30188:SF2 - ABC TRANSPORTER PERMEASE PROTEIN-RELATED // PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTAGGGTTTTAGGCTGATCCCACACCCGCACTTCGGATTTGGCCCTTG |
| Internal bar code: | TTATGCTTGCTACATCGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2069 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGGAGCTTCGTGAGGTG |
| Suggested primer 2: | GCAGCCATGACAATGCGTAG |