| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052531 |
| Chromosome: | chromosome 1 |
| Location: | 3906195 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025700 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAAACCCACCTATGGACATAATGCAGGCGCCGTTGAGGCCGTCGCTG |
| Internal bar code: | TAGTGCCTTGGTTCCGAACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2558 |
| LEAP-Seq percent confirming: | 75.9036 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCGGGCAATTTAGGACA |
| Suggested primer 2: | GGGGCGTCTTACCTTGAACA |