| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052538 |
| Chromosome: | chromosome 6 |
| Location: | 540641 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252401 | NMDA1 | (1 of 1) PTHR23291//PTHR23291:SF50 - BAX INHIBITOR-RELATED // PROTEIN LIFEGUARD 4; Conserved protein of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCAGACCGGCCGCGTGCCGGGGCCGCTCGAATGCGCTGGCCATCTGT |
| Internal bar code: | GTATTCGCGGACACAGCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3131 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAATTCCGGCTGTGTGG |
| Suggested primer 2: | CTGGATGCGCATTACACAGC |