Insertion junction: CLIP2.052576_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.052576
Chromosome:chromosome 9
Location:135758
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386700 kinesin-13,KIN13-1,CrKin13,KIN13A Kinesin motor protein 13; (1 of 1) K10393 - kinesin family member 2/24 (KIF2_24, MCAK) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGAAAATGCAGGACTATGCAGGCGGCAATCTGCCCCAACCCAACCCT
Internal bar code:CTAGTCTTTTACTAGACTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:174
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACGCGTTCAAGTTTGAC
Suggested primer 2:AGGTGGTGCTCAATGATGCA