| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052581 |
| Chromosome: | chromosome 5 |
| Location: | 2509366 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239200 | FAP275 | Flagellar Associated Protein 275 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGGGCAAGGGCGTAAGCCTTGGGATTGCCTGGTGAGGCATGCCAGGA |
| Internal bar code: | AGAGGTTTAGGCATGGCTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3653 |
| LEAP-Seq percent confirming: | 92.7536 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATGCTCCACCTGCTAAC |
| Suggested primer 2: | ACAAACCCACCTCACGTGTT |