Insertion junction: CLIP2.052595_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052595
Chromosome:chromosome 15
Location:960488
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639600 (1 of 781) IPR000104 - Antifreeze protein, type I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCGCCACTTGCCCCTGCTCCGCGACGTCCGCGAGCGCGGTCTCTTCA
Internal bar code:TGTTTTGTATCTTTTGCTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2973
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAACTCAACCAACGGCAC
Suggested primer 2:GCTGAGGGAAGGGAGGTCTA